Coding
EcAKR4-1-B

Part:BBa_K3332010:Design

Designed by: Xiaoyu Wang   Group: iGEM20_XMU-China   (2020-10-17)


GOX-BrkA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 294
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 513
    Illegal NgoMIV site found at 1243
    Illegal NgoMIV site found at 1672
    Illegal NgoMIV site found at 2308
    Illegal AgeI site found at 583
    Illegal AgeI site found at 660
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2276


Design Notes

We will choose the best anchor systerm.


Source

BrkA is from Bordetella pertussis and GOX is form Echinochloa colonum (L.) Link.

References